Expression of Poplar Chitinase in Tomato Leads to Inhibition
of Development in Colorado Potato Beetle |
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Authors: | Susan D Lawrence Nicole G Novak |
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Institution: | (1) United States Department of Agriculture, Agricultural Research Service, Insect Biocontrol Laboratory, BARC-West, Bldg. 011A, Room 214, Beltsville, MD 20705, USA |
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Abstract: | The previously described poplar chitinase, WIN6, is induced during infestation by gypsy moth (Lymantria dispar L.) larvae, thus suggesting a role in defense against insect pests. To test this hypothesis, we produced tomato seedlings
infected with a recombinant potato virus X (PVX), which produces WIN6, and tested its insecticidal properties on Colorado
potato beetle CPB; Leptinotarsa decemlineata (Say)], which is a serious pest of tomatoes and other crops. The advantage of PVX is that plant material is ready for insect
bioassay within 3–4 weeks of constructing the recombinant virus. Considering that production of transgenic tomato seedlings
using Agrobacterium takes at least 6 months, this hastens the rate at which genes can be examined. Upon insect bioassay, only 47% CPB neonates
feeding on leaves containing >0.3% w/w WIN6 developed to 2nd instar while 93% of controls reached 2nd instar. To our knowledge
this is the first plant chitinase that retards development of an insect pest.
Revisions requested 12 December 2005; Revisions received 18 January 2006 |
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Keywords: | chitinase herbivore insect resistance Lycopersicon esculentum Populus |
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