Induction of ovulation in <Emphasis Type="Italic">Xenopus</Emphasis>without hCG injection: the effect of adding steroids into the aquatic environment |
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Authors: | Aoi Ogawa Junpei Dake Yu-ki Iwashina Toshinobu Tokumoto |
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Institution: | (1) Department of Biology, Faculty of Science, National University Corporation Shizuoka University, 422-8529 Shizuoka, Japan |
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Abstract: | Background The African clawed frog, Xenopus laevis, is widely used in studies of oogenesis, meiotic cell cycle and early embryonic development. However, in order to perform
such studies, eggs are normally collected after the injection of hCG into the dorsal lymph sac of fully-grown female frogs
following pre-injection of PMSF. Although this protocol is established and used as standard laboratory approach, there are
some concerns over whether the injections could cause the transmission of deleterious microorganisms. Moreover, these injection
protocols require a competent skilled worker to carry out the procedure efficiently. |
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Keywords: | |
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