Canine distemper virus utilizes different receptors to infect chicken embryo fibroblasts and vero cells |
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Authors: | Jun Chen Xiu Liang Pei-fu Chen |
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Institution: | College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China |
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Abstract: | Inducing animal viruses to adapt to chicken embryos or chicken embryo fibroblasts (CEF) is a common method to develop attenuated
live vaccines with full security. Canine distemper virus (CDV) also does this, but the mechanisms and particular receptors
remain unclear. Virus overlay protein blot assays were carried out on CEF membrane proteins, which were extracted respectively
with a Mem-PER™ kit, a radioimmunoprecipitation assay buffer or a modified co-immunoprecipitation method, and revealed a common
57 kDa positive band that differed from the 42-kDa positive band in Vero cells and also from those receptors reported in lymphocytes
and 293 cells, indicating a receptor diversity of CDV and the possibility of the 57-kDa protein acting as a receptor that
is involved in adaptive infection of CDV Kunming strain to CEF. |
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Keywords: | Canine distemper virus(CDV) Cellular receptor Chicken embryo fibroblasts(CEF) Veto cells Virus overlay protein blot assay |
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