| 表达狂犬病毒糖蛋白的重组痘苗病毒的组建与鉴定 |
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| 引用本文： | 林枫,侯云德.表达狂犬病毒糖蛋白的重组痘苗病毒的组建与鉴定[J].病毒学报,1992,8(3):210-217. |
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| 作者姓名： | 林枫 侯云德 |
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| 作者单位： | 卫生部武汉生物制品研究所，卫生部武汉生物制品研究所，卫生部武汉生物制品研究所，中国预防医学科学院病毒所病毒基因工程国家重点实验室，Center for Disease Control，Atlanta，USA |
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| 摘 要： |
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| 关 键 词： | 重组痘苗病毒 狂犬病毒 糖蛋白 |
CONSTRUCTION AND CHARACTERISTIC OF VACCINIA VIRUS RECOMBINANTS EXPRESSING RABIESVIRUS GLYCOPROTEIN |
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| Lin Feng Zhu Jiahong Chen Naimin Hou Yunde Joseph Esposito .Wuhan Institute of Biological Products,Ministry of Public Health..CONSTRUCTION AND CHARACTERISTIC OF VACCINIA VIRUS RECOMBINANTS EXPRESSING RABIESVIRUS GLYCOPROTEIN[J].Chinese Journal of Virology,1992,8(3):210-217. |
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| Authors: | Lin Feng Zhu Jiahong Chen Naimin Hou Yunde Joseph Esposito Wuhan Institute of Biological Products Ministry of Public Health |
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| Institution: | Lin Feng Zhu Jiahong Chen Naimin Hou Yunde Joseph Esposito 1.Wuhan Institute of Biological Products,Ministry of Public Health. 2.Institute of Virology,Chinese Academy of Preventive Medicine 3.Centers for Disease Control,Atlanta,USA |
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| Abstract: | Two recombinants of vaccinia virus ( Tian Tan strain ) containing the coding sequence of Challenge Virus Standard ( CVS ) rabiesvirus glycopro tein were produced by insertion of the glycoprotein gene into the vaccinia tk gene. The coding sequence was controled under the vaccinia early -late promoter p7.5. The recombinants differed slightly in its sequence upstream of the translation start codon and both express effectively mature glycoprotein of rabiesvirus, which was transported to the cell membrane.Immunological assays and trial immunizations in animals showed that the molecular weight of non-fusion glycoprotein of rabiesvirus approximates 64kD. It react specifically with antibody against authentic glycoprotein of rabiesvirus. Each recombinant induced rabiesvirus neutralizing antibodies in mice and rabbits and protection against lethal street virus challenge. |
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| Keywords: | Recombinant vaccinia virus Olycoprotein of rabiesvirus Thymidine kinase gene |
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