p16基因甲基化状态与散发性大肠癌的相关性研究

为探讨p1 6基因甲基化状态与散发性大肠癌发生发展的关系 ,用甲基化特异性的聚合酶链反应 (methylati omspecificPCR ,MSP)结合测序检测散发性大肠癌及相应癌旁组织p1 6基因甲基化状态。研究发现p1 6基因在散发性大肠癌中甲基化率为 2 8 9% (1 3 4 5 ) ,有 8例癌及癌旁组织都发生了甲基化 ;有淋巴结及远处转移的甲基化率为5 0 % (8 1 6 ) ,高于无转移的甲基化率 2 0 8(5 2 4 ) (P <0 0 5 )。p1 6基因高甲基化是散发性大肠癌中常见的分子改变之一 ,大肠癌中p1 6基因高甲基化可能发生在癌变早期并与大肠癌的恶性进展有相关性

Relationship Between CpG Methylation of p16 Gene and Sporatic Colorectal Cancer

In order to the relationship between CpG methylation of p16 gene and histological features of sporatic colorectal cancer,methylation specific PCR(MSP) and DNA sequencing was used to analyze the methylation status of p16 tumor suppressor gene in 45 sporatic colorectal cancers and corresponding normal tissues.Among the 45 colorectal cancers, p16 hypermethylation was detected in 13 cases(28 9%),in the 13 methylated cases,8 cases were hypermethylated in cancers and tissues adjacent to the cancer.Hypermethylation of p16 gene was more frequent (8/16,50%) in cancers with metastatic lymph node than those without metastasis (5/24,20 8%) ( P <0 05).Hypermethylation of p16 gene is a frequent molecular event in sporatic colorectal cancer;it may occur at the early stage of the cancer and is related to invasion and metastasis of colorectal cancer.