Purification,crystallization and preliminary crystallographic studies of a class II chitinase from kidney bean seeds |
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Authors: | Zhou Zhaocai Liu Hanjian Li Yikun Gong Weimin |
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Institution: | Key Laboratory of Structural Biology of Chinese Academic of Sciences, School of Life Sciences, University of Science & Technology of China, Hefei, Anhui 230026, The People's Republic of China. |
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Abstract: | A new member of class II chitinase from Phaseolus vulgaris was purified and crystallized. Diffraction data to 2.7A resolution have been collected and the preliminary crystallographic studies have been completed. The space group is P1 with unit cell parameters of a=36.32A, b=46.24A, c=70.36A, a =97.9 degrees, b=103.8 degrees and g =110.5 degrees. Molecular replacement and initial refinement statistics indicate there are two chitinase molecules in the crystallographic asymmetric unit. |
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