Phase II enzyme induction by a carotenoid,lutein, in a PC12D neuronal cell line |
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| Authors: | Seiji Miyake Saori Kobayashi Kazuo Tsubota Yoko Ozawa |
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| Institution: | 1. Laboratory of Retinal Cell Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan;2. Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan;3. Wakasa Seikatsu Co., Ltd., 134 Chudoujiminami-cho, Shimogyo-ku, Kyoto 600-8813, Japan |
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| Abstract: | The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina. |
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| Keywords: | PC12D rat adrenal pheochromocytoma cell line ROS reactive oxygen species Nrf2 nuclear factor-like 2 HO-1 oxygenase-1 SOD1 and 2 superoxide dismutase 1 and 2 Prdx1 peroxiredoxin 1 NQO1 NAD(P)H dehydrogenase quinone 1 |
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