Measurement of aberrant glycosylation of prostate specific antigen can improve specificity in early detection of prostate cancer |
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Authors: | Tohru Yoneyama Chikara Ohyama Shingo Hatakeyama Shintaro Narita Tomonori Habuchi Takuya Koie Kazuyuki Mori Kazuya IPJ Hidari Maho Yamaguchi Takashi Suzuki Yuki Tobisawa |
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Institution: | 1. Department of Urology, Hirosaki University Graduate School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan;2. Department of Urology , Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan;3. Department of Food and Nutrition, Junior College Division, University of Aizu, 1-1 Aza Kadota, Oaza Yahata, Ikki-machi, Aizuwakamatsu 965-0003, Fukushima, Japan;4. Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Suruga-ku, Shizuoka 442-8002, Shizuoka, Japan |
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Abstract: | IntroductionWe previously identified prostate cancer (PCa)-associated aberrant glycosylation of PSA, where α2,3-linked sialylation is an additional terminal N-glycan on free PSA (S2,3PSA). We then developed a new assay system measuring S2,3PSA using a magnetic microbead-based immunoassay. We compared the diagnostic accuracy of conventional PSA and percent-free PSA (%fPSA) tests.MethodsWe used MagPlex beads to measure serum S2,3PSA levels using anti-human fPSA monoclonal antibody (8A6) for capture and anti-α2,3-linked sialic acid monoclonal antibody (HYB4) for detection. We determined the cutoff values in a training test and measured serum S2,3PSA levels in 314 patients who underwent biopsy, including 138 PCa and 176 non-PCa patients with PSA of <10.0 ng/ml. Serum S2,3PSA levels were presented as mean fluorescence intensity (MFI). Receiver operating characteristic curves were used to evaluate the diagnostic accuracy of total PSA, %fPSA, and S2,3PSA.ResultsWe determined an MFI cutoff value of 1130 with a sensitivity of 95.0% and specificity of 72.0% for the diagnosis of PCa in the training test. In the validation study, the area under the curve for the detection of PCa with S2,3PSA was 0.84, which was significantly higher than that with PSA or %fPSA.ConclusionsAlthough the present study is small and preliminary, these results suggest that the measurement of serum S2,3PSA using a magnetic microbead-based immunoassay may improve the accuracy of early detection of PCa and reduce unnecessary prostate biopsy. |
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Keywords: | Prostate specific antigen Cancer associated aberrant glycosylation Immunoassay α2 3-linked sialylation |
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