Up-regulation of eEF1A2 promotes proliferation and inhibits apoptosis in prostate cancer |
| |
Authors: | Yue Sun Chengli Du Bo Wang Yanling Zhang Xiaoyan Liu Guoping Ren |
| |
Institution: | 1. Department of Pathology, The First Affiliated Hospital, Zhejiang University Medical College, Hangzhou, China;2. Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China |
| |
Abstract: | BackgroundeEF1A2 is a protein translation factor involved in protein synthesis, which possesses important function roles in cancer development. This study aims at investigating the expression pattern of eEF1A2 in prostate cancer and its potential role in prostate cancer development.MethodsWe examined the expression level of eEF1A2 in 30 pairs of prostate cancer tissues by using RT-PCR and immunohistochemical staining (IHC). Then we applied siRNA specifically targeting eEF1A2 to down-regulate its expression in DU-145 and PC-3 cells. Flow cytometer was used to explore apoptosis and Western-blot was used to detect the pathway proteins of apoptosis.ResultsOur results showed that the expression level of eEF1A2 in prostate cancer tissues was significantly higher compared to their corresponding normal tissues. Reduction of eEF1A2 expression in DU-145 and PC-3 cells led to a dramatic inhibition of proliferation accompanied with enhanced apoptosis rate. Western blot revealed that apoptosis pathway proteins (caspase3, BAD, BAX, PUMA) were significantly up-regulated after suppression of eEF1A2. More importantly, the levels of eEF1A2 and caspase3 were inversely correlated in prostate cancer tissues.ConclusionOur data suggests that eEF1A2 plays an important role in prostate cancer development, especially in inhibiting apoptosis. So eEF1A2 might serve as a potential therapeutic target in prostate cancer. |
| |
Keywords: | Prostate cancer (PCa) eEF1A2 Proliferation Apoptosis Caspase3 |
本文献已被 ScienceDirect 等数据库收录! |
|