| Targeted Genome Editing by Recombinant Adeno-Associated Virus （rAAV） Vectors for Generating Genetically Modified Pigs |
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| 引用本文： | Luo Y,Kofod-Olsen E,Christensen R,S?rensen CB,Bolund L.Targeted Genome Editing by Recombinant Adeno-Associated Virus （rAAV） Vectors for Generating Genetically Modified Pigs[J].遗传学报,2012,39(6):269-274. |
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| 作者姓名： | Luo Y Kofod-Olsen E Christensen R Sørensen CB Bolund L |
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| 作者单位： | [1]Department of Biomedicine, Aarhus University, Wilhelm Meyers AUe 4, DK-8000 Aarhus C, Denmark [2]BGI/HuaDa Jigin-Shenzhen, Shenzhen 510813, China |
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| 基金项目： | the,Danish,National,Research,Infrastructure,Programme,to,the,Danish,Genetically,Modified,Animal,Resource,(DAGMAR),the,"Sino-Danish,Breast,Cancer,Research,Centre",under,the,auspices,of,the,Danish,National,Research,Foundation,(Grundforskningsfonden),the,National,Natural,Science,Foundation,of,China |
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| 摘 要： | Recombinant adeno-associated virus(rAAV) vectors have been extensively used for experimental gene therapy of inherited human diseases.Several advantages,such as simple vector construction,high targeting frequency by homologous recombination,and applicability to many cell types,make rAAV an attractive approach for targeted genome editing.Combined with cloning by somatic cell nuclear transfer(SCNT),this technology has recently been successfully adapted to generate gene-targeted pigs as models for cystic fibrosis, hereditary tyrosinemia type 1,and breast cancer.This review summarizes the development of rAAV for targeted genome editing in mammalian cells and provides strategies for enhancing the rAAV-mediated targeting frequency by homologous recombination.We discuss current development and application of the rAAV vectors for targeted genome editing in porcine primary fibroblasts,which are subsequently used as donor cells for SCNT to generate cloned genetically designed pigs and provide positive perspectives for the generation of gene-targeted pigs with rAAV in the future.
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| 关 键 词： | rAAV Gene targeting Homologous recombination SCNT Genetically modified pigs |
| 收稿时间： | 8 May 2012 |
Targeted genome editing by recombinant adeno-associated virus (rAAV) vectors for generating genetically modified pigs |
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| Luo Yonglun,Kofod-Olsen Emil,Christensen Rikke,S?rensen Charlotte Brandt,Bolund Lars.Targeted genome editing by recombinant adeno-associated virus (rAAV) vectors for generating genetically modified pigs[J].Journal of Genetics and Genomics,2012,39(6):269-274. |
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| Authors: | Luo Yonglun Kofod-Olsen Emil Christensen Rikke Sørensen Charlotte Brandt Bolund Lars |
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| Institution: | Department of Biomedicine, Aarhus University, Wilhelm Meyers Alle 4, DK-8000 Aarhus C, Denmark. alun@hum-gen.au.dk |
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| Abstract: | Recombinant adeno-associated virus (rAAV) vectors have been extensively used for experimental gene therapy of inherited human diseases. Several advantages, such as simple vector construction, high targeting frequency by homologous recombination, and applicability to many cell types, make rAAV an attractive approach for targeted genome editing. Combined with cloning by somatic cell nuclear transfer (SCNT), this technology has recently been successfully adapted to generate gene-targeted pigs as models for cystic fibrosis, hereditary tyrosinemia type 1, and breast cancer. This review summarizes the development of rAAV for targeted genome editing in mammalian cells and provides strategies for enhancing the rAAV-mediated targeting frequency by homologous recombination. We discuss current development and application of the rAAV vectors for targeted genome editing in porcine primary fibroblasts, which are subsequently used as donor cells for SCNT to generate cloned genetically designed pigs and provide positive perspectives for the generation of gene-targeted pigs with rAAV in the future. |
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| Keywords: | rAAV Gene targeting Homologous recombination SCNT Genetically modified pigs |
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