Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix |
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| Authors: | Yi Shao Luowa Quyang Yueping Zhou Jing Tang Yuehui Tan Qiuping Liu Zhirong Lin Tingting Yin Fangfang Qiu Zuguo Liu |
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| Institution: | (1) Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology of Sun Yat-sen University, Guangzhou, Guangdong Province, China;(2) Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China;(3) Affiliated Zhongshan Hospital of Xiamen University, Xiamen, Fujian Province, China;(4) The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China;(5) Eye Institute of Xiamen University, No. 168 Da Xue Road, Xiamen, 361005, People’s Republic of China |
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| Abstract: | This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized
matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation)
and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine
fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that
PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds
that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using
acellular porcine cornea. |
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