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Membrane microheterogeneity: Förster resonance energy transfer characterization of lateral membrane domains |
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| Authors: | Luís M S Loura Fábio Fernandes Manuel Prieto |
| Institution: | 1. Faculdade de Farmácia, Universidade de Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal 2. Centro de Química de évora, Rua Rom?o Ramalho, 59, 7000-671, évora, Portugal 3. Department of Membrane Biophysics, Max-Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, G?ttingen, Germany 4. Centro de Química Física Molecular and Institute of Nanosciences and Nanotechnologies, Complexo I, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisbon, Portugal |
| Abstract: | Lateral membrane heterogeneity, in the form of lipid rafts and microdomains, is currently implicated in cell processes including signal transduction, endocytosis, and cholesterol trafficking. Various biophysical techniques have been used to detect and characterize lateral membrane domains. Among these, Förster resonance energy transfer (FRET) has the crucial advantage of being sensitive to domain sizes smaller than 50-100 nm, below the resolution of optical microscopy but, apparently, similar to those of rafts in cell membranes. In the last decade, several formalisms for the analysis of FRET in heterogeneous membrane systems have been derived and applied to the study of microdomains. They are critically described and illustrated here. |
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