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A highly specific radioimmunoassay for progesterone using antibodies covalently linked to arylamine-glass particles
Authors:F H Bodley  A Chapdelaine  G Flickinger  G Mikhail  S Yaverbaum  K D Roberts
Affiliation:Departments of Biochemistry and of Medicine, University of Montreal and Maisonneuve-Rosemont Hospital, Montreal., Quebec, Canada
Abstract:Antibody was produced in sheep following the administration of the antigen, progesterone-11α-succinyl-BSA. After treatment with Rivanol, the protein of the albumin-free antiserum was covalently bound to arylamine glass particles using glutar-aldehyde as the coupling agent. This antibody-glass preparation was used for measurement of plasma progesterone by radioimmunoassay. Progesterone was extracted from human plasma with petroleum ether. Of those Steroids which are petroleum ether extractable, only pregnenolone exhibited minimal cross-reactivity. Immobilization of the antibody by attachment to an insoluble support allows the separation of free from antibody-bound steroid without the use of absorbents, such as florisil and charcoal.
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