抗消毒剂型微生物培养基研究

二氧化氯、过氧乙酸、次氯酸钠及过氧化氢等消毒剂高浓度可以杀灭微生物，低浓度可以抑制微生物生长。在微生物检测中，要消除消毒剂的干扰，培养基的抗消毒剂指数必须控制在１２．０—２４.５之间。消毒剂解抑剂Ⅰ的抗消毒剂指数为 １２．０，对细菌和真菌生长无明显抑制作用，加入经改良和优化的普通培养基后，制得５种抗消毒剂型培养基，在灭菌前后和一年的保存期内，其抗消毒剂指数基本保持不变。当采用大样倾注平板法和液体大样法检测残留消毒剂的样品时，使用抗消毒剂型培养基检出细菌和真菌能力大大高于普通培养基。

RESEARCHES ON ANTI-DISINFECTANT MICROBIAL MEDIA

The microorganisms could be killed by the high concentration disinfectants such as chlorine dioxide, peroxyacetic acid, sodium hypochlorite and hydrogen peroxide, and the microbial growth could be inhibited by the low concentration disinfectants. So, the microbial detectability could be disturbed seriously by the disinfectants remained in the samples. In order to eliminate the disinfectant disturbance on microbial detection, the anti-disinfectant indexes(ADI)must be controlled between 12.0-24.5. The index of the disinfectant removing reagent(DRR) I was 12.0, it did not inhibit obviously the bacterial and fungous growth in the plates. The five kinds of the anti-disinfectant media(ADM) could be made up by adding DRR I to the improved and optimized general media. After the media were disinfected or in one year storage life, their ADI could be almost kept stable. When the big sample pouring plate method and the liquid big sample method were applied to detect the samples with the remains of the disinfectants, the bacterial and fungous detectability of the anti-disinfectant media was much higher than that of the general microbial media.