Poly(a)+ rna during vegetative development ofacetabularia peniculus |
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Authors: | I Mine K Okuda D Menzel |
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Institution: | 1. Department of Biology, Faculty of Science, Kochi University, Kochi 2. Department of Biology, Faculty of Science, Kochi University, 2-5-1 Akebono-cho, 780-8520, Kochi, Japan 3. Abteilung Zellbiologie der Pflanzen, Botanisches Institut und Botanischer Garten, Rheinische Friedrich-Wilhelms-Universit?t Bonn, Bonn
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Abstract: | Summary In the juvenile stage, the diploid giant-celled green algae Acetabularia spp. are differentiated into an upright stalk and
an irregularly branched rhizoid. Early amputation and grafting experiments as well as biochemical and molecular analyses have
shown that mRNA (as poly(A)+ RNA) is continuously supplied from the primary nucleus in the rhizoid and accumulates in the stalk apex. In the present study,
localization of poly(A)+ RNA in the juvenile stage of theAcetabularia peniculus was investigated by fluorescent in situ hybridization using oligo(dT) as a probe. The signal was localized in the apical
cytoplasm and, in addition, multiple longitudinal striations throughout the stalk and rhizoid cytoplasm. A large portion of
the poly(A)+ RNA striations exhibited structural polarity, broadened at one end and gradually thinned toward the other end. Some of the
striations in the rhizoid cytoplasm were continuous with a zone of signal in the area of the perinuclear rim. The poly(A)+ RNA striations were associated with thick bands of longitudinal actin bundles which run through the entire length of the
stalk. Cytochalasin D caused fragmentation of the actin bundles and irregular distribution of the fluorescent signal. We suggest
that the poly(A)+ RNA striations constitute a hitherto unknown form of packaged mRNA that is transported over large distances along the actin
cytoskeleton to be stored and expressed in the growing apex. |
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Keywords: | Acetabularia peniculus Actin cytoskeleton Dasycladaceae Fluorescent in situ hybridization Poly(A)+ RNA Polyphysa peniculus |
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