ETB-type receptors mediate endothelin-stimulated contraction in the aortic vascular smooth muscle of the spiny dogfish shark,Squalus acanthias

Using various agonists, and the specific antagonist BQ-123, we have examined the sensitivity to endothelin of the vascular smooth muscle of the ventral aorta of the spiny dogfish shark, Squalus acanthias. Human endothelin-1 produced significant contraction of isolated vascular smooth muscle rings, with an EC₅₀ of 10 nmol·1^-1. The presence of an intact endothelium did not alter this response but the magnitude of the contraction was greater in rings with an intact endothelium. The response to 0.2 mol·1^-1 endothelin-1 was equivalent to that of 0.1 mmol·1^-1 acetylcholine, and significantly greater than that to 80 mmol·1^-1 KCl, suggesting high sensitivity even to the heterologous, mammalian peptide. The Hill plot of the contractile response was a straight line with a slope of 1.12, indicating that a single receptor was mediating the response. Endothelin-1, endothelin-3, and sarafotoxin S6c produced similar concentration-response curves, and the response to endothelin-1 was insensitive to the ET_A-specific inhibitor BQ-123. Our data are consistent with the hypothesis that the receptor involved in the contractile response to endothelin of shark aortic vascular smooth muscle is of the ET_B-rather than the ET_A-type.Abbreviations ACh acetylcholine - ANP atrial natriuretic peptide - CA celiacomesenterie artery - CRC concentration response curve - DMSO dimethylsulphoxide - ET endothelin - STX sarafotoxin - VSM vascular smooth muscle - EDCF endothelium derived contraction factor