RFLP analysis for species separation in the generaBipolaris andCurvularia |
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Authors: | Minoru Nakada Chihiro Tanaka Koichiro Tsunewaki Mitsuya Tsuda |
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Institution: | (1) Pesticide Research Institute, Faculty of Agriculture, Kyoto University, Sakyoku, 606 Kyoto, Japan;(2) Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Sakyoku, 606 Kyoto, Japan |
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Abstract: | Restriction fragment length polymorphism (RFLP) of the total DNA ofBipolaris andCurvularia species was analysed using arbitrarily chosen genomic clones of DNA fromCurvularia lunata andBipolaris maydis as probes. Clear differences among species in both genera, resulting in different banding positions, were obtained with some probe-enzyme combinations. Intraspecific polymorphism in banding positions with these probe-enzyme combinations was slight. These analyses allow discrimination between the species. DNA fingerprinting with intrageneric probes is a potentially useful tool for species separation and identification inBipolaris andCurvularia when coupled with another characteristic such as conidial morphology.Curvularia aeria comb. nov. was proposed forCurvularia lunata var.aeria on the basis of differences in RFLP banding patterns and differences in conidial morphology. |
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Keywords: | Bipolaris Curvularia Curvularia aeria comb nov genomic DNA RFLP analysis |
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