A simple method of generating axenic derivatives of Dictyostelium strains |
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Authors: | Alastair Morrison Adrian Harwood |
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Abstract: | We describe a generally applicable method of adapting Dictyostelium from growth on a bacterial food source to axenic growth. Cells are initially selected by growth on a plastic substratum but subsequently acquire the ability to grow in suspension culture. These strains can be transformed efficiently by DNA-mediated gene transfer. |
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