ROSO: optimizing oligonucleotide probes for microarrays |
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| Authors: | Reymond Nancie Charles Hubert Duret Laurent Calevro Federica Beslon Guillaume Fayard Jean-Michel |
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| Affiliation: | Biologie Fonctionnelle, Insectes et Interactions (BF2I), UMR INRA/INSA de Lyon, 69621 Villeurbanne, France. nancie.reymond@jouy.inra.fr |
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| Abstract: | ![]()
ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set. AVAILABILITY: The program is available on the internet. Sources are freely available, for non-profit use, on request to the authors. Supplementary information: http://pbil.univ-lyon1.fr/roso |
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