Participation of hup gene product in ori2-dependent replication of fertility plasmid F |
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Authors: | Morimasa Wada Kyoko Kohno Fumio Imamoto and Yasunobu Kano |
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Institution: | Laboratory of Molecular Genetics, (Riken) Tsukuba Life Science Center, The Institute of Physical and Chemical Research, Tsukuba, Ibaraki 305, Japan |
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Abstract: | The fertility plasmid F'gal was not stably maintained in a hupA-hupB double mutant of Escherichia coli. Moreover, mini-F plasmids pFZY1, pFTC1 and pFTC2 were unable to transform the double mutant, though these plasmids efficiently transformed cells harboring a hupA or hupB single mutation. The composite plasmid pFHS1, which consists of the f5 DNA fragment of F plasmid and the whole DNA of a pSC101 derivative that carries a temperature-sensitive mutation for DNA replication, was not stably maintained in the hup double mutant at 42°C. These findings strongly suggest that HU protein is required for ori2-dependent replication of the F plasmid. |
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Keywords: | Escherichia coli mini-F plasmid replicon F'gal pSC101 hupA-hupB mutant HU protein recombinant DNA |
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