芜菁原生质体的分离及绿色荧光蛋白的瞬时表达

目的：分离芜菁叶片原生质体,建立蛋白质在芜菁原生质体的瞬时表达系统。方法：以津田芜菁成叶为试材,酶解分离原生质体;通过PEG介导的转化,将编码绿色荧光蛋白（GFP）的瞬时表达载体转入原生质体中,用激光扫描共聚焦显微镜检测原生质体中GFP的表达情况。结果：分离出大量的津田芜菁原生质体,并获得了较高的转化效率,GFP在整个原生质体中都有表达。结论：建立了津田芜菁原生质体瞬时表达系统。

Protoplast Isolation of Brassica rapa 'Tsuda' Turnip and Transient Expression of Green Fluorescent Protein

Objective：To isolate protoplasts of leaves in Brassica rapa ‘Tsuda’ and to build the system of transient expression of protein in protoplasts.Methods：The leaves of ‘Tsuda’ turnip were used as materials to isolate protoplast by enzyme digestion.The transient expression vector with reported gene coded green fluorescent protein（GFP） was transferred into protoplast by PEG method,and the expression of GFP in protoplast was examined by laser scanning confocal microscopy.Results：It showed that many protoplasts of leaves were isolated and high efficiency of transformation was obtained.The protein of GFP was examined to express in all over the protoplast.Conclusion：The system of transient expression of target gene in protoplasts of B.rapa ‘Tsuda’ has been established.