| 激光、血卟啉对肿瘤细胞DNA单链断裂及其重接的影响 |
| |
| 引用本文： | 夏寿萱,徐惠英,范国才.激光、血卟啉对肿瘤细胞DNA单链断裂及其重接的影响[J].中国生物化学与分子生物学报,1986,2(4):65-70. |
| |
| 作者姓名： | 夏寿萱 徐惠英 范国才 |
| |
| 作者单位： | 放射医学研究所 北京
(夏寿萱，徐惠英)，放射医学研究所 北京(范国才) |
| |
| 摘 要： | 用简化的Kohn氏碱洗脱法,观察了光敏剂血卟啉衍生物(HPD)对小鼠S-180肿瘤细胞DNA单链断裂及其重接修复的影响。激光HPD能导致S-180细胞DNA单链断裂明显增加,而且这种断裂随着保温时间的延长,继续增多。在本实验条件下没有观察到HPD对X线的增敏作用,HPD不能增加X线所致的DNA单链断裂,也不能影响其重接。单链断裂重接动力学的实验进一步证明了这个论点。
|
| 关 键 词： | 血卟啉 DNA损伤和修复 DNA单链断裂 光敏作用 |
| 收稿时间： | 1986-08-20 |
THE EFFECT OF LASER AND HEMATOPORPHYRIN DERIVATIVES ON THE DNA SINGLE STRAND BREAKS AND THEIR REJOINING IN TUMOR CELLS |
| |
| Xia,Shou-xuan Xu,Hui-ying Fan,Guo-cai.THE EFFECT OF LASER AND HEMATOPORPHYRIN DERIVATIVES ON THE DNA SINGLE STRAND BREAKS AND THEIR REJOINING IN TUMOR CELLS[J].Chinese Journal of Biochemistry and Molecular Biology,1986,2(4):65-70. |
| |
| Authors: | Xia Shou-xuan Xu Hui-ying Fan Guo-cai |
| |
| Institution: | (Institute of Radiation Medicine,Beijing |
| |
| Abstract: | By means of modified Kohn's alkaline elution method,the photosensiti-zation effect of hematoporphyrin derivatives (HPD) on the DNA damage and repair in tumor cells was investigated.Mouse tumor cells in suspension were irradiated with He-Ne laser in the presence of HPD.Increased amount of single strand breaks (SSB) in cellular DNA were found immediately after irradiation.If the irradiated cells were incubated at 37℃ for 30 min.,no SSB rejoining was observed,moreover the damage was enhanced.This phenomenon was quite diffe-rent from that observed in cells irradiated with X ray in the presence of HPD.These results provided further support to our previous suggestions that after laser-HPD treatment and followed by incubation,primary lesions as well as secondary processes leading to the enhancement of injuries were induced in the irradiated tumor cells. |
| |
| Keywords: | Hematoporphyrin DNA damage and repair DNA strand breaks Photosensitization |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
