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Primary culture of chicken hepatocytes in serum-free medium (pH 7.8) secreted albumin and transferrin for a long period in free gas exchange with the atmosphere |
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| Authors: | Makoto Fujii Izumi Yoshino Mituru Suzuki Teturoh Higuchi Satoru Mukai Takayoshi Aoki Takao Fukunaga Yasushi Sugimoto Yuichi Inoue Jun Kusuda Takeyori Saheki Masako Sato Seiichi Hayashi Masahiko Tamaki Tukasa Sugano |
| Institution: | a Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, Kagoshima 890, Japan b Division of Genetic Resoures, National Institute of Health, Toyama 1-23-1, Shinjuku-ku, Tokyo, 620, Japan c Department of Biochemistry, Faculty of Medicine, Kagoshima University, Usuki 1208-1, Kagoshima 890, Japan d Laboratory of Nutrition, Faculty of Education, Kagoshima University, Kagoshima 890, Japan e Faculty of Fisheries, Kagoshima University, Kagoshima 890, Japan f Faculty of Agriculture, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753, Japan g Department of Veterinary Physiology, University of Osaka Prefecture, Sakai, Osaka 591, Japan |
| Abstract: | To study the liver functions of chicken, we examined the primary culture of chicken hepatocytes, and found an easy method of long-term culture with free atmosphere exchange. Chicken hepatocytes were obtained by collagenase perfusion and cultured at 37°C as a monolayer without substratum in serum-free L-15 medium (pH 7.8) with free atmosphere exchange. The amounts of albumin and transferrin in medium were assayed by ELISA. The culture of chicken hepatocytes was maintained in the serum-free L-15 medium (pH 7.8) at 37°C with free atmosphere exchange for 20 days. The amount of albumin secreted in the medium decreased to low levels early in culture; however, this was followed by marked increase from day 9 to day 17 of culture. The amount of transferrin was constant until day 6, then it too increased considerably with further culture. We reported an easy method for the simple monolayer culture of chicken hepatocytes in serum-free L-15 medium (pH 7.8) with free atmosphere exchange over an extended period. Expression of liver-specific functions, viz. albumin and transferrin synthesis, was observed after 1 week of culture. |
| Keywords: | Chicken Hepatocyte Culture Albumin Transferrin |
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