SD大鼠酒精性脂肪肝模型的监测分析

目的：通过血清生化指标和病理学的监测分析来建立标准的SD大鼠酒精性脂肪肝动物模型。方法：选取40只SD大鼠，随机分为两组，模型组采用直接饮酒法，于第8、12和20周时检测大鼠血清生化指标：丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、甘油三酯（TG），并于第8、12周时随机采集5只大鼠肝组织，20周时采集剩余所有大鼠肝组织并进行病理学分析。结果：模型组于第8、12和20周时体重增长量均低于对照组（P〈0．01），血清ALT、AST均高于对照组（P〈0．01），第8周和12周时TG高于对照组（P〈0．01）。病理学结果显示肝组织从8周至20周呈现出酒精性脂肪肝、重度酒精性脂肪肝伴肝炎和酒精性肝纤维化等演变过程。结论：直接饮酒法可成功地复制出酒精性脂肪肝动物模型，通过监测分析可了解酒精性脂肪肝病变的整个过程，为今后建立酒精性脂肪肝和肝纤维化动物模型提供了理论参考。

Monitoring Analysis of Alcoholic Fatty Liver Model in SD Rats

Objective： To establish a standard SD rat model of alcoholic fatty liver through monitoring analysis of blood serum biochemical indicator and liver pathology. Methods： 40 SD rats were selected and divided into two groups randomly. Rats in model groups directly drunk ethanol for 20 weeks. At the end of 8th week, the 12th week and the 20th week, the blood serum biochemical indicators （ALT, AST and TG） and liver pathology were examined. Results： Compared with the control group, weight increase was significant lower, the ALT and AST were significantly higher in model group at the end of 8th week, the 12th week and the 20th week （P〈0.01）. At the end of 8th week and the 12th week, the TG were higher than those of the control model （P〈0.01）. Pathological results showed liver tissue developed fxom alcoholic fatty liver, severe alcoholic fatty liver with hepatitis to alcoholic hepatic fibrosis from 8th week to 20th week. Conclusions： Direct drinking ethanol can be successfully replicated animal model of alcoholic fatty liver, and we can understand about the process about alcoholic fatty liver through monitoring analysis, then this model can provide a theoretical reference for the future establishment of alcoholic fatty liver and alcoholic hepatic fibrosis animal model.