红景天苷对LPS诱导的HSC-T6细胞增殖作用及机制研究

目的：观察红景天苷（Salidroside，Sal）对大鼠肝星状细胞（hepaticstellatecell，HSC）一T6增殖的影响，并探讨一氧化氮（NO）在此过程中的作用。方法：选择脂多糖（LPS）活化HSC—T6，采用不同浓度的Sal，作用于经LPS活化的大鼠HSC-T6，24小时后进行如下实验：噻唑兰（MTT）比色法检测HSC—T6增殖；NO荧光探针（DAF-FMDA）检测细胞内NO浓度；Westem-blot检测INOS蛋白水平。结果：在LPS作用于HSC．T6细胞24小时后，与对照组相比，细胞增殖增加（P〈0．01），在Sal作用下，HSC-T6细胞增殖与LPS组相比受到抑制（P〈0．01），并呈浓度依赖性；在Sal处理HSC．T6细胞24小时后，细胞内NO水平有所上升，并呈浓度依赖性增加（P〈O．01）；Sal预处理后的HSC—T6细胞，iNOS蛋白表达有所升高（P〈0．01）。结论：Sal对HSC—T6细胞的增殖具有浓度依赖性的抑制作用，NO可能是抑制增殖的因素之一。

Study on Effects and Mechanism of Salidroside on Proliferation of Lipopolysaccharide-induced Hepatic Stellate Cells

Objective： To investigate the effect of Salidroside on proliferation of hepatic stellate Cells （HSC） and estimate the function of the NO during the procedure. Methods： Choosing the lipopolysaccharide （LPS）used to activate HSC-T6. Rat HSC-T6 activated by LPS were incubated with various concentration of Sal. Then conduct the following experiment after twenty-four hours： MTT colorimetric assay was used to detect the Proliferation of HSC-T6; The intracellular NO was detected using DAF-FM DA. Western blot were used to investigate the expression of iNOS protein. Results： Compared with the control group, the ratio of cell proliferation increased （P〈0.01） after LPS-induced HSC-T6 for 24h. Compared with the LPS group, the ratio of cell proliferation decreased（P〈0.01） after Sal treatment with LPS-induced HSC-T6 for 24h, and in a dose-dependent manner. In group treated with Sal, the NO concentration and expression level ofiNOS in HSC-T6 increased （P〈0.01）, and in a dose-dependent mariner（P〈0.01）. Conclusion： Sal could inhibit the proliferation of HSC-T6, which could be mediated by NO partially at least.