移植BMSCs对乳腺炎大鼠血清中自由基和抗氧化酶水平变化影响的研究

目的：探讨乳房基底部移植大鼠骨髓间充质干细胞（BMSCs）对实验性乳腺炎大鼠血清中自由基和抗氧化酶水平变化的影响。方法：将70只SD雌性大鼠,其中的60只随机分为3组：对照组、抗生素组和移植BMSCs组,每组20只。通过乳头管灌注内毒素的方法建立实验性大鼠乳腺炎模型,对照组基底部注射生理盐水;抗生素组基底部注射抗生素（5000 U/侧）;移植BMSCs组乳房基底部移植BMSC 50μL（1×106个）,试验0 d时灌注内毒素,1 d时注射BMSCs、抗生素及生理盐水,-1、0、3、5、7 d分别采血样,制备血清,检测血清中丙二醛（MDA）、一氧化氮（NO）、超氧化物歧化酶（SOD）、谷胱甘肽氧化还原酶（GSH-Px）、过氧化氢酶（CAT）水平。结果：移植BMSC组能显著降低乳腺炎大鼠血清中7 d时MDA、NO水平（P〈0.05）,且MDA水平显著低于抗生素组（P〈0.05）;移植BMSCs组7 d时血清中GSH-Px水平和1、3、5 d时CAT水平显著高于对照组和抗生素组（P〈0.05）。结论：BMSC能显著降低乳腺炎大鼠血清中后期NO和MDA水平,提高血清中前期CAT和后期GSH-PX,从而提高机体清除自由基的能力。

The Study on Free Radicals and Antioxidant Enzyme Level in the Serum of Mastitis Model Rats after Injecting Bone Marrow Mesenchymal Stem Cells

Objective：To investigate the effects on serum free radicals and antioxidants enzyme levels of experimental mastitis that transplanted rat marrow mesenchymal stem cells（BMSCs） through the breast base. Methods： 5 rats selected from 70 normal female SD rats were slaughter after 72 h of postpartum namely test-1 d, at the same time the rest of 65 rats were perfused 0.05 mL（50μg） endotoxin through the nipple tube as the reference to domestic common way, 5 rats that the data obtained from these rats would be equally compared and contrasted with data from other groups later were randomly selected and slaughter after 1 d namely test 0 d that established experimental mastitis model successfully. At the same the rest of the 60 rats were randomly divided into three groups： control group（injection of saline in breast base, 50 μL）, antibiotic group injection of antibiotic in breast base, 50 μL,（50 00 U / side）] and BMSCs transplantion group（injection of BMSCs in breast base μL（1×106）], n=20, and slaughter 5 rats each group at 1 d,3 d,5 d,7 d respectively,cardiac blood was collected from the dead bodies and preserved for detecting serum Malondialdehyde（MDA）, nitric oxide（NO）,superoxide dismutase（SOD）, glutathione oxidation reductase（GSH-Px）, hydrogen peroxide enzyme（CAT）. Results： The results showed that, BMSC transplantation group could significantly lower the level of MDA、NO in serum and level of MDA was significantly lower than antibiotic group at 7 d（P 0.05）, while the level of GSH-Px at 7 d and CAT at1,3,5 d of BMSCs transplantation group were significantly higher than control group and antibiotic group（P 0.05）. Conclusions： BMSCs can lower the late levels of MDA and NO,improve prophase level of CAT and late level of GSH-Px, thereby enhancing the body＇s ability to scanenge free radicals.