UMSCs对U87MG细胞增殖的影响

目的：通过对研究脐带间充质干细胞（Umbilical cord mesenchymalstellcells，UCMSCs）与人恶性胶质母细胞瘤细胞U87MG细胞（U87 Malignant glioma cells）体外共培养，模拟肿瘤生长的内环境，以及其对U87MG细胞增值作用的影响及肿瘤细胞与间充质干细胞的共培养方法。方法：提取人脐带间充质干细胞进行体外培养、扩增，用MTT法测定uMSCS上清液对U87MG的影响，用瑞士染色法检测U87MG形态学变化。结果：MTT比色法结果显示UMSCS对U87MG有抑制作用。96小时培养后1：8、1：4、1：2及未稀释的UMSCs上清液对u87MG的抑制率分别为17％，24％，37．2％及46．4％，u87MG细胞形态亦随着培养时间的延长由多角形变为梭形，突起消失，细胞间骨架结构断裂。结论：通过对共培养前后U87MG与UMSCs共培养后形态学变化、生长曲线变化及对生长周期的影响作用的观察分析，得出UMSCs及其上清液对U87MG有抑制作用，而且呈时间及浓度依赖性。

Effects of Umbilical Cord Mesenchymal Stell Cells on the Proliferation of U87 Malignant Glioma Cells

Objective： To study umbilical cord mesenchymal stem cells （Umbilical cord mesenchymal stell cells, UCMSCs） human malignant glioblastoma cell tumor cells U87MG cells （U87 Malignant glioma cells） in vitro co-culture simulate tumor growth within the environment, and their impact onU87MG cell proliferation effect of tumor cells with mesenchymal stem cells co-cultured. Method： Eextraction of human umbilical cord mesenchymal stem cells cultured in vitro amplification determined by MTT UMSCS supematant of U87MG, Switzerland staining U87MG morphological changes. Results： MTT colorimetric the results show UMSCS of U87MG inhibi- tion. U87MG inhibition rate of the 1：8,1：4,1：2 and not diluted UMSCs of supematant after 96 hours of incubation were 17%, 24%, 37.2% and 46.4%, U87MG cell morphology as the culture time extend fi＇om polygonal to spindle-shaped protrusions disappear cell skeleton structure fi＇acture. Conclusion： Morphological changes after co-culture before and after U87MG and UMSCs of co-culture, the growth curve and the observation and analysis of the effect of the growth cycle, draw UMSCs its supematant inhibition of U87MG, and in a time and concentration dependence.