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Different CMS sources found in Beta vulgaris ssp maritima: mitochondrial variability in wild populations revealed by a rapid screening procedure
Authors:P Saumitou-Laprade  G J A Rouwendal  J Cuguen  F A Krens  G Michaelis
Institution:(1) Laboratory of Genetics and Evolution of Plant Populations, URA CNRS 1185, Scientific and Technical University of Lille, F-59655 Villeneuve d'Ascq CEDEX, France;(2) Institute of Agriculture and Food Industry (IAAL), University of Lille 1, F-59655 Villeneuve d'Ascq CEDEX, France;(3) Agrotechnical Research Institute (ATO-DLO), Haagsteeg 6, P.O. Box 17, NL-6700 AA Wageningen, The Netherlands;(4) Center for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, NL-6700 AA Wageningen, The Netherlands;(5) Botanical Institute of the University of Düsseldorf, Universitätsstrasse 1, W-4000, 1, FRG
Abstract:Summary Mitochondrial DNA (mtDNA) variation in natural Beta maritima populations has been characterized by way of Southern blot hybridizations of total DNA using non-radioactive probes and chemiluminescent detection. It was found that the previously described N (ldquonormalrdquo) mitochondrial type could be subdivided into three subtypes. A new mitochondrial genotype (type R) was distinguished in addition to the previously described type S. Both are male-sterile cytoplasms and can produce a. segregation of sexual phenotypes in their progenies depending on the nuclear background. The populations contained at least two to four different mitochondrial genotypes.
Keywords:CMS  Wild beet  Cultivated sugar beet  RFLP  MtDNA variability
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