RAPD analysis of somaclonal variants derived from embryo callus cultures of peach |
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| Authors: | G Hashmi R Huettel R Meyer L Krusberg F Hammerschlag |
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| Institution: | (1) Plant Molecular Biology and Nematology Laboratories USDA/ARS, 20705 Beltsville, MD, USA;(2) Department of Botany, University of Maryland, 20742 College Park, MD, USA;(3) Nematology Laboratories, USDA/ARS, 20705 Beltsville, MD, USA;(4) Department of Botany, University of Maryland, 20742 College Park, MD, USA;(5) Plant Molecular Biology, USDA/ARS, 20705 Beltsville, MD, USA;(6) Present address: Department of Entomology, Rutgers University, P.O. Box 231, 08903 New Brunswick, NJ, USA;(7) Present address: USDA/CREES, Aerospace Builfing, 220215 Washington, DC, USA;(8) Present address: USDA/APHIS, PPQ, Building 580, BARC-E, 20705 Beltsville, MD, USA;(9) Present address: USDA/ARS, Fruit Laboratory, Building 010A, BARC-W, Beltsville, MD, USA |
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| Abstract: | Peach Prunus persica (L.) Batsch] regenerants from cv  Sunhigh embryo no. 156, regenerants obtained from cv Redhaven embryo no. 30, and two peach cultivars Sunhigh and Redhaven, were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with up to 60 10-mer primers. Although 35 primers produced results with scoreable bands, only 10 of the primers revealed polymorphism for regenerants of embryo no. 156 and cv Sunhigh, and 1 revealed a low level of polymorphism for regenerants of embryo no. 30 and cv Redhaven. This study demonstrates the feasibility of using RAPD markers to identify somaclonal variants of peach and provides evidence for the existence of genetic differences among these variants.Abbreviations
PCR
Polymerase chain reaction
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RAPD
random amplified polymorphic DNA
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RFLP
Restriction fragment length polymorphism
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CTAB
cetyltrimethyl ammonium bromide
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PVP
polyvinyl pyrolidone
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dNTP
deoxy-ribonucleotide triphosphate
Communicated by R. N. Trigiano |
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| Keywords: | Prunus persica Somaclonal variation |
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