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Site-specific antibodies to the erbB oncogene product immunoprecipitate epidermal growth factor receptor |
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| Authors: | T Akiyama Y Yamada H Ogawara N Richert I Pastan T Yamamoto M Kasuga |
| Affiliation: | 1. Second Department of Biochemistry, Meiji College of Pharmacy, Nozawa-1, Setagaya-ku Tokyo 154, Japan;2. Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205 USA;3. Laboratory of Oncology, Institute of Medical Science, University of Tokyo, 4-6-1 Shiroganedai, Minato-ku Tokyo 108, Japan;4. Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo Tokyo 113, Japan |
| Abstract: | Antiserum to a defined region (residues 373-383) of the erbB oncogene product immunoprecipitated a 170,000 dalton protein that was phosphorylated in an EGF-sensitive fashion as well as the 125I-EGF-receptor complex from A431 human epidermoid carcinoma cells. Preincubation of the antiserum with an excess of the synthetic peptide corresponding to the defined region blocked the immunoprecipitation of this protein. A partial proteolytic peptide map of this immunoprecipitated 170,000 dalton protein was identical to that of the authentic EGF receptor. These results suggest immunological similarity between the erbB gene product and the EGF receptor. |
| Keywords: | ANF atrial natriuretic factor SSC 0.15 M NaCl 0.015M sodium citrate pH7 Denhardt's solution 0.02% each polyvinylpyrrolidone Ficoll bovine serum albumin |
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