| 侧孢芽孢杆菌质粒提取方法的探究 |
| |
| 引用本文： | 金术超,杜春梅,平文祥,关洪宇.侧孢芽孢杆菌质粒提取方法的探究[J].生物技术,2005,15(6):44-46. |
| |
| 作者姓名： | 金术超 杜春梅 平文祥 关洪宇 |
| |
| 作者单位： | 黑龙江大学微生物重点实验室,黑龙江,哈尔滨,150080 |
| |
| 基金项目： | 黑龙江省教育厅骨干教师科研基金（1054G037）;黑龙江省研究生创新科研资金项目. |
| |
| 摘 要： | 以能够降解有机磷农药的两株侧孢芽孢杆菌BL-21和BL-22为研究对象，分别采用碱裂解法、试剂盒提取法和SDS法对侧孢芽孢杆菌BL-21和BL-22的质粒进行提取，并通过凝胶电泳和紫外分光光度法对提取结果进行分析，试验结果证明，适合侧孢芽孢杆菌BL-21和BL-22的质粒提取方法是SDS裂解法，该方法提取的质粒大小为10kb，且该方法提取的结果稳定，质粒的产量和质量均符合分子生物学实验的要求。
|
| 关 键 词： | 质粒 侧孢芽孢杆菌 提取方法 |
| 文章编号： | 1004-311X（2005）06-0044-03 |
| 收稿时间： | 2005-05-28 |
| 修稿时间： | 2005-08-19 |
Comparative Study on the Method of Extracting Plasmid DNA of Bacillus laterosporus |
| |
| JIN Shu-chao,DU Chun-mei,PING Wen-xiang,GUAN Hong-yu.Comparative Study on the Method of Extracting Plasmid DNA of Bacillus laterosporus[J].Biotechnology,2005,15(6):44-46. |
| |
| Authors: | JIN Shu-chao DU Chun-mei PING Wen-xiang GUAN Hong-yu |
| |
| Institution: | Key Lab. of Mieroorganism, Heilongjiang University, Harbin 150080, China |
| |
| Abstract: | The plasmids of Bacillus laterosporus BL-21 and BL-22 were extracted by three different methods.The plasmids were analyzed by agarose gel electrophoresis and ultra spectrophotometers.The results showed that method of SDS extraction was the best method to extract the plasmid from Bacillus laterosporus.The size of plasmids was 10kb.Experiments demonstrated that consequences from this protocol were stable,the quality and yield of plasmid DNA produced from it had met the standard for many molecular hiology experiments. |
| |
| Keywords: | plasmid Bacillus laterosporus method of extraction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
