Enhanced production of cellulases byCellulomonas strains grown on different cellulosic residues |
| |
Authors: | M I Rajoka K A Malik |
| |
Institution: | (1) National Institute for Biotechnology and Genetic Engineering, P.O.Box 577, Faisalabad, Pakistan |
| |
Abstract: | Cellulomonas strains consumed commercial cellulose, cellulosic residues, xylan, cellobiose and carboxymethyl cellulose (CMC) as carbon
sources in liquid culture, the growth being the most on cellobiose medium. All three components of the cellulase complex ofCellulomonas were produced when the organisms utilized all substrates as sole carbon and energy sources. The filter-paper cellulase (FPase)
and endo-glucanase (CMCase) activities were higher in media containing α-cellulose and cellulosic residues than in media containing
CMC, cellobiose, and xylan. Cell-free supernatants of all organisms exhibited greater CMC hydrolyzing activity than filter
paper and β-glucoside hydrolyzing activities. All strains synthesized β-glucosidase maximally on cellobiose followed by commercial
cellulose and cellulosic residues.C. biazotea produced the highest FPase and CMCase activity during growth on α-cellulose. It was followed byC. flavigena, C. cellasea, andC. fimi. Endo-glucanase and FPase from all organisms were secreted into the medium; 10–13 % became adsorbed on the surface of the
insoluble substrates and could be successfully eluted using Tween 80. β-Glucosidase was located in cell extracts from all
organisms.C. biazotea produced FPase and β-glucosidase activities several-fold greater than those produced by many other strains ofCellulomonas and some other cellulolytic bacteria and fungi.
These studies were supported byPakistan Atomic Energy Commission. Some chemicals were purchased from funds allocated byUnited States Agency for International Development, Washington (DC, USA), under PSTC proposal 6.163. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|