Practical Approaches to Low Density Lipoprotein Oxidation: Whys, Wherefores and Pitfalls |
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Authors: | Catherine Rice-evans David Leake K Richard Bruckdorfer Anthony T Diplock |
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Institution: |
a Free Radical Research Group, Division of Biochemistry and Molecular Biology, United Medical and Dental Schools of Guy's and St Thomas's Hospitals, London
b School of Animal and Microbial Sciences, University of Reading, Reading
c Department of Biochemistry, Royal Free Hospital School of Medicine, London |
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Abstract: | The purpose of this review is to bring together the different approaches for studying the oxidation of low density lipoproteins and try to identify some critical factors which will permit greater comparability between laboratories. These issues are discussed both in terms of the variety of exogenous mediators of oxidation applied (transition metal ions, haem proteins, azo initiators, peroxynitrite, cells etc.) and their raisons d'etre, as well as the methodologies (formation of conjugated dienes, hydroperoxides, decomposition products of lipid peroxidation, altered surface charge, macrophage uptake) applicable to the different stages of the oxidation and the factors underlying their accurate execution and interpretation. |
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Keywords: | Oxidized LDL lipid hydroperoxide conjugated diene apoprotein B-100 |
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