不同剂量MOG_(35-55)抗原诱导EAE小鼠模型的免疫组织化学比较

Comparision of Immunohistochemical Changes in C57BL/6 Mouse Models with Experimental Autoimmune Encephalomyelitis Induced with Different Doses of Myelin Oligodendrocyte Glycoprotein35-55

Objective To compare the conditions to induce experimental autoimmune encephalomyelitis（EAE）in C57BL /6 mice with myelin oligodendrocyte glycoprotein35-55 and the pathological characteristics revealed by immunohisto-chemistry.Methods Fifty female SPF C57BL /6 mice 6-8 weeks old were divided randomly into four groups：normal group and three EAE model groups（high-dose group,middle-dose group and low-dose group）.The mice of the three model groups were injected subcutaneously over flanks with the antigen containing 300,150,50 μg MOG35-55 /mouse and completeFreund＇s adjuvant（CFA） in the same ratio,respectively.500 ng pertussis toxin（PTX） in 200 μL physiological saline was given i.p.to the mice of the three model groups on the day of immunization and 48 h later.Physiological saline was given to the normal group instead.The effects of different doses of MOG35-55 on the body weight,incidence,mortality rate and neu-rological scores of C57BL /6 mice were observed.Meanwhile,the pathological changes of CNS were examined by hematoxy-lin-eosin（HE） staining and the expression of Olig2,MBP and GFAP by immunohistochemical staining.Results The C57BL /6 mouse model of EAE was successfully induced with three different doses of MOG35-55.The EAE model represen-ted a chronic,monophasic feature of disease.There were inflammatory cell infiltration around small veins called sleeve-like changes and demyelination in the brain and spinal cord of mice.However,the influence of low-dose of MOG35-55 on loss of weight,neurological score and pathological changes as well as the expression of Olig2,MBP and GFAP seemed to be more significant than that in other model groups.Conclusion An immune-induced mouse model of EAE has been successfully established with MOG35-55,especially with a low dose of 50 μg.This EAE mouse model is stable,with a high incidence and low mortality rate,and can be used in research of multiple sclerosis（MS）.