Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea) |
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| Authors: | Wei Wei Haizhen Wu Hongyan Xu Xiao Zhang Teng Chu Changwen Wu Kangmei Chang Yuanxing Zhang |
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| Institution: | 1. Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata 990-9585, Japan;2. Department of Molecular Patho-Biochemistry and Patho-Biology, Yamagata University School of Medicine, Yamagata 990-9585, Japan;3. Department of Obstetrics and Gynecology, Toyama University, Graduate School of Medical Sciences, Toyama 930-0194, Japan;4. Department of Plasma Proteins, Sanquin Research, Amsterdam, the Netherlands;5. Department of Experimental Vascular Medicine, Academic Medical Center, University of Amsterdam, the Netherlands |
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| Abstract: | Complement component 1 inhibitor (C1INH) is a crucial protein in controlling activation of many plasma mediator pathways and can directly interact with Gram negative bacteria. The full-length cDNA of lycC1INH gene was identified from the large yellow croaker. It is of 2046 nucleotides (nt) encoding a protein of 599 amino acids, with a 5′-untranslated region of 99 nt and a 3′-untranslated region of 147 nt including the poly (A) tail. The deduced protein contains a C-terminal serpin (serine protease inhibitor) domain, and two N-terminus immunoglobulin domains without significant homology to other species. Western blot analysis of the protein expression showed that the expression of lycC1INH was obviously up-regulated in liver, spleen and head kidney of the fish challenged by attenuated live Vibrio anguillarum strain. This indicated that lycC1INH might be involved in the immune response of large yellow croaker to bacterial challenge. |
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