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Determination of ribonuclease activity in coloured extracts of citrus leaves
Authors:E Hellín  A Torrecillas  F Sevilla  S Llorente  C F Alcaraz
Institution:(1) CSIC, Centro do Edafología y Biología Aplicada del Segura, Avda. de la Fama, 1, P.O. Box 195, 30003 Murcia, Spain
Abstract:A rapid method is presented for the determination of ribonuclease activity in coloured extracts from citrus leaves. At the same time, the influence exercised by several precipitating reagents and the storage time at 4 °c on the activity of the enzymatic system are studied. In addition the enzyme stability against heat is studied.
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