体细胞介导制备转基因动物的新途径

体细胞介导制备转基因动物的技术路线是在核移植技术的基础上建立起来的。它的问世不过只有四五年时间 ,但显示了其具有广泛的应用前景。本综述比较了不同制备转基因动物技术的优缺点 ,介绍了体细胞介导制备转基因动物的基本技术路线 ,着重阐述了体细胞转染和整合细胞的克隆 ,以及体细胞介导的转基因技术的局限性和应用前景

Somatic Cell-Mediated Transgene:A New Route for Making Transgenic Animals

The technique route for making transgenic animals by somatic cell mediated transgene has been developed based on the technique of nuclear transfer. Although it came into being for only about 4-5years, it has shown broad application prospect and great potential. In this review, we compared advantages and disadvantages of different methods for making transgenic animals, expounded the basic technique route for making transgenic animals by somatic cell mediated transgene, emphasized the key points of somatic cell transfection and cloning of integrated cells, and the limitation and potential application of this technique.