Synthesis and matrix assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry study of phosphopeptide |
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| Authors: | Luo Shi-Zhong Li Yan-Mei Chen Zhong-Zhou Abe Hiroshi Cui Li-Ping Nakanishi Hiroshi Qin Xu-Rong Zhao Yu-Fen |
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| Affiliation: | (1) Bioorganic Phosphorus Chemistry Laboratory Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing, P.R. China;(2) Biological Information Research Center, National Institute Advanced Industry Science and Technology AIST, Central 6, 1-1, Higashi, Tsukuba, Japan |
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| Abstract: | ![]()
Summary The symmetry phosphoramidite reagents were synthesized via a two-step route and used for the ‘one pot’ synthesis of the phosphoserine building block Fmoc-Ser(PO(OBzl)OH)-OH. The procedure is simple and rapid, and product is obtained in high yield. When using this building block, the phosphopeptide (RKGS(PO3H2)SSNEPSSDSLSSPTLLAL) related to the C-terminal of c-Fos protein was synthesized manually by Fmoc/t-Bu procedure and characterized by matrix assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry. In the fragment ion of phosphopeptide in the MALDI-TOF mass spectrometry with a curved field reflection, the acquired information can be used to identify the sequences and the phosphorylation sites of the peptide. |
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| Keywords: | building block curved field reflection matrix assisted laser desorption/ionization time of flight one pot phosphopeptide solid-phase peptide synthesis |
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