Little divergence in ribosomal DNA internal transcribed spacer -1 and -2 sequences among non-digitate species of Laminaria (Phaeophyceae) from Hokkaido, Japan |
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| Authors: | Norishige Yotsukura Takashi Denboh Taizo Motomura Takeo Horiguchi Annette W. Coleman Terunobu Ichimura |
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| Affiliation: | Institute of Algological Research, Faculty of Science, Hokkaido University, Muroran, Hokkaido 051–0003, Japan;;Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060–0810, Japan;Department of Molecular and Cellular Biology and Biochemistry, Brown University, Providence, RI 02912, USA. |
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| Abstract: | ![]()
The nuclear ribosomal DNA internal transcribed spacer (ITS-1 and ITS-2) sequences were determined for 10 of 12 Japanese non-digitate Laminaria species, Kjell-maniella gyrata (Kjellman) Miyabe, Costaria costata (Turner) Saunders, Alaria praelonga Kjellman and Chorda filum (L.) Stackhouse collected at Hokkaido. Phyloge-netic analyses (maximum parsimony and distance matrix) of these sequences, including published data for L. sac-charina (L.) Lamouroux from Canada, showed strong nucleotide conservation among these species of Laminaria, but two phylogenetically distinct species groups were recognized. A L. japonica group encompassing L. yapon/ca Areschoug, L. religiosa Miyabe, L. ochotensis Miyabe, L. diabolica Miyabe, L. longipedalis Okamura, L. angustata Kjellman and L. longissima Miyabe; and a L. saccharina group including L. coriacea Miyabe, L. sac-charina, L. cichorioides Miyabe and L. yendoana Miyabe. As to other laminarialean genera, Kjellmaniella gyrata was most closely related to the genus Laminaria, being related to the second Laminaria species group based on both parsimony and distant tree values. |
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| Keywords: | internal transcribed spacer-1 internal transcribed spacer-2 kelp Laminaria molecular phylogeny species problem |
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