Correlation of the cytotoxic activity of four different alkaloids, from Chelidonium majus (greater celandine), with their DNA intercalating properties and ability to induce breaks in the DNA of NK/Ly murine lymphoma cells |
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Authors: | Vitaliy O Kaminskyy Maxim D Lootsik Rostyslav S Stoika |
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Institution: | (1) Ivan Franko Lviv National University, 79005 Lviv, Ukraine;(2) Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine |
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Abstract: | The purpose of this study was to examine the relationship between the DNA intercalating characteristics and the DNA damaging
capacity of four alkaloids extracted from Chelidonium majus L, as well as their toxicity towards murine NK/Ly lymphoma cells. Chelerythrine, sanguinarine and coptisine were found to be
intercalated into the DNA isolated from NK/Ly cells, meanwhile, chelidonine exhibited no affinity to DNA. Sanguinarine exhibited
the greatest toxicity toward NK/Ly cells, and the toxicity of the other three decreased in descending order: chelerythrine,
coptisine and chelidonine. Chelerythrine and sanguinarine caused DNA damage, illustrated by the formation of comets of the
third class. Coptisine was less toxic than chelerythrine and sanguinarine, and affected the formation the same class of comets
in higher concentration. The quantity of comets induced by chelidonine were negligible, a finding consistent with its inability
to intercalate into DNA structure. The ability of four main alkaloids of Chelidonium majus L., to intercalate into DNA isolated from murine NK/Ly lymphoma cells, correlated with their ability to induce breaks in cellular
DNA and with their toxic effect towards those cells. |
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Keywords: | Chelidonium majus L alkaloids NK/Ly lymphoma cytotoxicity DNA intercalation methyl green displacement test comet assay |
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