Environmental conditions differentially affect vir gene induction in different Agrobacterium strains. Role of the VirA sensor protein |
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| Authors: | S. C. J. Turk L. S. Melchers H. den Dulk-Ras A. J. G. Regensburg-Tuïnk P. J. J. Hooykaas |
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| Affiliation: | (1) Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, Netherlands;(2) Present address: MOGEN International NV, Einsteinweg 97, 2333 CB Leiden, Netherlands |
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| Abstract: | The induction of vir gene expression in different types of Agrobacterium strains shows different pH sensitivity profiles. The pH sensitivity pattern demonstrated by octopine Ti strains was similar to that of a supervirulent leucinopine Ti strain, whereas this was different from that shown by nopaline Ti strains and agropine Ri strains. Data are given which indicate that these differences are due to different properties of the virA genes of these wild types. An exceptional case was formed by strains with the limited-host-range plasmid pTiAG57 which showed AS-dependent vir induction only if reduced inoculum sizes were used and the temperature was 28°C or below. |
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| Keywords: | Agrobacterium virulence gene VirA protein gene regulation sensor protein |
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