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| 扁蓿豆育成品系遗传多样性的RAPD研究 | |
| 引用本文： | 赵丽丽,王照兰,杜建材,胡卉芳,李青丰,张彦艳. 扁蓿豆育成品系遗传多样性的RAPD研究[J]. 西北植物学报, 2009, 29(12) |
| 作者姓名： | 赵丽丽 王照兰 杜建材 胡卉芳 李青丰 张彦艳 |
| 作者单位： | 1. 内蒙古农业大学,生态环境学院,呼和浩特,010019;中国农业科学院,草原研究所,农业部草原资源与生态重点开放实验室,呼和浩特,010010 2. 中国农业科学院,草原研究所,农业部草原资源与生态重点开放实验室,呼和浩特,010010 3. 内蒙古农业大学,生态环境学院,呼和浩特,010019 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金，牧草育种技术研究及产业开发，国家重点基础研究发展计划，中国农科院2008年度院科研基金资助 |
| 摘 要： | 用 RAPD分子标记对4个扁蓿豆育成品系进行遗传多样性分析.结果表明:筛选的12条多态性RAPD引物共检测出126个等位基因位点,平均每条引物检测到10.5个位点,多态位点比率占94.4%.扁蓿豆品系的Nei's遗传多样性指数(H)为0.266 2,Shannon多样性指数(I)为0.414 5.Nei's指数估算和分子方差分析(AMOVA)均证实扁蓿豆品系的遗传多样性主要存在于品系内,品系间有一定的基因交流(基因流为2.756 7).聚类结果表明,品系00-61和00-81遗传差异相对较小,品系90-36与前两者之间的遗传差异较大,品系93-21与各品系的遗传差异最大. |
| 关 键 词： | 扁蓿豆 遗传多样性 聚类分析 主坐标分析 |
Genetic Diversity of Medicago ruthenica Srains Based on RAPD Markers |
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| ZHAO Li-li,WANG Zhao-lan,DU Jian-cai,Hu Hui-fang,LI Qing-feng,ZHANG Yan-yan. Genetic Diversity of Medicago ruthenica Srains Based on RAPD Markers[J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(12) | |
| Authors: | ZHAO Li-li WANG Zhao-lan DU Jian-cai Hu Hui-fang LI Qing-feng ZHANG Yan-yan |
| Affiliation: | ZHAO Li-li1,2,WANG Zhao-lan2,DU Jian-cai1,Hu Hui-fang2,LI Qing-feng1,ZHANG Yan-yan2(1 College of Ecology , Environmental Science,Inner Mongolia Agricultural University,Huhhot 010019,China,2 Grassl, Research Institute of Chinese Academy of Agricultural Science,Huhhot 010010,China) |
| Abstract: | Four Medicago ruthenica breeding strains were studied for the analysis of genetic diversity by RAPD markers.Twelve effective primers were screened and totally 126 alleles were detected.The average number of alleles amplified by per primer was 10.5,and the polymorphism percentage was 94.4%.The result indicated that Nei's gene diversity(H) was 0.266 2,Shannon information index(I) was 0.414 5.Nei's analysis and AMOVA analysis both indicated that the majority genetic variation of M.ruthenica strains occurred wi... |
| Keywords: | RAPD Medicago ruthenica RAPD genetic diversity cluster analysis principal coordinate analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |