Cellular Responses to Mechanical Stress: Invited Review: Effects of flow on vascular endothelial intracellular calcium signaling of rat aortas ex vivo |
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Authors: | Jen Chauying J; Jhiang Shuo-Ju; Chen Hsiun-Ing |
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Abstract: | To study the effects of flow on in situendothelial intracellular calcium concentration(Ca2+]i) signaling, rat aortic rings wereloaded with fura 2, mounted on a tissue flow chamber, and divided intocontrol and flow-pretreated groups. The latter was perfused with bufferat a shear stress of 50 dyns/cm2 for 1 h. EndothelialCa2+]i responses to ACh or shear stresseswere determined by ratio image analysis. Moreover, ACh-inducedCa2+]i elevation responses were measured ina calcium-free buffer, or in the presence of SKF-96365, to elucidatethe role of calcium influx in the flow effects. Our results showed that1) ACh increased endothelialCa2+]i in a dose-dependent manner, and theseresponses were incremented by flow-pretreatment; 2) thedifferences in ACh-induced Ca2+]i elevationbetween control and flow-pretreated groups were abolished by SKF-96365or by Ca2+-free buffer; and 3) in the presenceof 105 M ATP, shear stress induced dose-dependentCa2+]i elevation responses that were notaltered by flow-pretreatment. In conclusion, flow-pretreatment augmentsthe ACh-induced endothelial calcium influx in rat aortas ex vivo. |
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