天然产物莪术醇单克隆抗体的制备

为制备小分子化合物莪术醇的单克隆抗体,先将莪术醇(curcumol)与载体蛋白牛血清蛋白(BSA)偶联形成完全抗原,用基质辅助激光解吸飞行时间质谱法(MALDI-TOF-MS)鉴定莪术醇人工抗原的偶联率,然后采用杂交瘤技术获得杂交瘤株,并对其进行小鼠腹水的制备与纯化.结果表明:莪术醇半抗原与载体的偶联比为19.6,单克...

Formation of monoclonal antibody against a major natural product curcumol

To get monoclonal antibody(mAb)against crude drug curcumol,curcumol-carrier protein conjugates were synthesized by the method that curcumol was conjugated with BSA.The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry(MALDI-TOF-MS).A hybridoma secreting monoclonal antibody against curcumol was produced by hybridoma technology,then preparation and purification of curcumol mAb use mice hybridoma ascitic fluid.Results showed that the ratio of hapten and bovine serum album was 19.6,the ELISA titers in the ascite fluids of the curcumol mAb were 1:51 200,ELISA analysis also proved that the curcumol mAbs reacted specifically with curcuoml antigen,polyacrylamide gel electrophoresis((SDS-PAGE)assay showed that the curcumol heavy chain was 5.0×104 and the light chain was 2.5×104.Conclusion the specific anti-curcumol mAbs were prepared,which would lay a theoretical foundation for detection and qulity control and immunoassay of curcuma kwangsiensis in Guangxi producing areas by Immunoassay.