首页 | 本学科首页   官方微博 | 高级检索  
     


DNA analysis of tropical plant species: An assessment of different drying methods
Authors:Stephen A. Harris
Affiliation:(1) School of Biological and Medical Sciences, Sir Harold Mitchell Building, University of St Andrews, St Andrews, KY16 9TH Fife, Great Britain
Abstract:Use of molecular techniques to study plant systematics has been restricted to either largely temperate groups or groups from which seed is readily obtained. Dried material has advantages over other methods of preservation for molecular analyses; it is cheap, easily undertaken, overcomes the difficulties associated with seed recalcitrance and the absence of seeding plants in the field, and most taxonomists are familiar with the method. Three drying techniques were assessed using material collected in the tropics. No differences in either the quantity or the quality of DNA extracted from material dried by these methods was detected. The implications of using dried material in molecular analyses are discussed.
Keywords:Leguminosae  Rubiaceae  Vochysiaceae  Leucaena  Vochysia  Qualea  Coffea  Molecular analysis  drying methods  ribosomal DNA  restriction analysis
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号