噬菌体展示技术筛选bFGF模拟短肽

目的：通过噬菌体展示技术筛选得到与FGFR结合的bFGF模拟短肽，为bFGF肽类抑制剂的研发提供实验基础。方法：以Balb/c 3T3细胞为靶标，以COS-7细胞作消减，对噬菌体随机七肽库进行4轮生物淘洗，再采用ELISA检测单克隆噬菌体对Balb/c 3T3亲和性和特异性，选取阳性克隆进行DNA测序分析。结果：从富集的噬菌体中获得12个阳性克隆，获得一组疏水性七肽及共同基序PR。结论：利用肽类新药开发的重要工具--噬菌体展示技术，得到2段bFGF的受体结合模拟肽，可望作为bFGF抑制剂的先导肽。

Selection of bFGF mimic peptide by phage display

Objective: To obtain the bFGF mimic peptide binding to FGFR via phage display, and to provide the base for developing peptide agonist of bFGF. Methods: Using Balb/c 3T3 cells as the target cells and COS-7 cells as the subtractive panning, the phage display heptapeptide library was biopanned for 4 rounds to obtain the single phage clones. The affinity and the specificity of the clones were assessed by ELISA. DNA sequencing was applied to further analyze the positive clones. Results: Twelve positive clones were selected from the enriched phages. A group of hydrophobic peptides containing a conserved motif, PR, was identified. Conclusion: Two bFGF mimic heptapeptides binding to FGFR were selected, which may be used as the candidates for bFGF agonist.