利用植物激素调控嫁接形成的初步研究

利用黄瓜（Ｃｕｃｕｍ ｉｓｓａｔｉｖｕｓ）试管苗进行离体茎段自体嫁接，研究ＩＢＡ 和６－ＢＡ 对嫁接形成的影响时发现：进行离体茎段嫁接时，用试管苗茎段可简化嫁接过程，减少污染。嫁接茎段的颜色变化、不定根发生和愈伤组织形成与激素浓度有关。植物激素通过影响砧木和接穗间维管束桥形成的时间和数目调控嫁接组合的发育。在作者的实验中，最佳的激素条件是：在接穗培养基中加ＩＢＡ １．２ ｍ ｇ／Ｌ，在接穗和砧木培养基中加６－ＢＡ ０．３ ｍ ｇ／Ｌ。

PRELIMINARY STUDIES ON CONTROLLING GRAFT UNION THROUGH PLANT HORMONES

The influences of IBA and 6 BA on the formation of grafting were studied by using explanted internode autografting of cucumber (Cucumis sativus) cultured in vitro, which was a simpler procedure for grafting with lower chance of contamination than the ordinary explanted internode grafting. Colour changes, root initiation and callus formation of the explanted internode graf related to the concentration of plant hormones added to the medium. Plant hormones controlled the formation of graft unions through influencing the time and the number of vascular bridges formation between the stock and the scion. In authors' experiments, the optimal condition of plant hormone control was achieved when IBA 1.2 mg/L was added in the scion medium and 6 BA 0.3 mg/L was added to scion and stock medium.