Hemin-induced Erk1/2 activation and heme oxygenase-1 expression in human umbilical vein endothelial cells |
| |
Authors: | Chen Nan Shao Wei Lv Pingping Zhang Shuluo Chen Yingying Zhu Li Lu Yuan Shen Yueliang |
| |
Affiliation: | a Department of Physiology, Zhejiang University School of Medicine, Hangzhou, Chinab Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, PR Chinac Department of Gastroenterology, Putuo People's hospital of Zhoushan, Zhejiang, China |
| |
Abstract: | ![]() Hemin has been reported to be protective in the pathological process, but its protective mechanisms have not been precisely defined. Hemin could induce Erk1/2 phosphorylation in astrocyte. Erk1/2 phosphorylation has been proved to be involved in many growth signals cellular transduction. However, little study has been conducted as to the relationship between hemin and Erk1/2 activation in human umbilical vein endothelial cells (HUVECs). The present study aimed to investigate the relationship between hemin and Erk1/2 phosphorylation in HUVECs. The results showed that low concentration of hemin induced and sustained phosphorylation of Erk1/2 for a long time. The HO inhibitor protoporphyrin IX zinc (II) abrogated phosphorylation of Erk1/2 induced by hemin. Biliverdin, one of the metabolites of hemin, obviously induced the Erk1/2 phosphorylation in HUVECs. Both hemin and biliverdin promoted HUVEC cell growth. The results strongly suggested that hemin could induce and sustain Erk1/2 phosphorylation in HUVECs by way of HO-1 induction and biliverdin produced from HO-1 catalysing hemin degradation. |
| |
Keywords: | Endothelial cells hemin Erk |
本文献已被 InformaWorld PubMed 等数据库收录! |
|