Selective removal of seedling root hairs for studies of the Rhizobium-legume symbiosis |
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| Authors: | David L Gerhold Frank B Dazzo Peter M Gresshoff |
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| Institution: | 1. Department of Microbiology and Public Helath, Michigan State University, East Lansing, MI 48824, U.S.A.;2. Department of Botanu, Australian National University, Canberra City, A.C.T. 2600, Australia |
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| Abstract: | A freeze-fracture method has been developed for the selective removal of root hairs from white clover (Trifolium repens L.) and alfalfa (Medicago sativa L.) seedling. This procedure yields sufficient material for analysis of root hair proteins by polyacrylamide gel electrophoresis and can be adapted to study in vivo protein synthesis in these differentiated epiderman cells. Clover root hairs which have been injected by the nitrogen-fixing symbiont, Rhizobium trifolii 0403, are also detached from roots by this process, yielding appropriate material to study root responses to the bacterial symbiont during the infection process. |
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| Keywords: | Medicago sativa Rhizobium Trifolium repens To whom all correspondence should be addressed |
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