Modulation of lyso-platelet activating factor:acetyl-CoA acetyltransferase from rat splenic microsomes. The role of cyclic AMP-dependent protein kinase |
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Authors: | Julián Gómez-Cambronero Susana Velasco JoséM Mato Mariano Sánchez-Crespo |
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Institution: | 1. Nefrología Experimental, Universidad Autónoma de Madrid, Reyes Católicos 2, Madrid 28040 Spain;2. Metabolismo, Nutrición y Hormonas, Fundación Jiménez Díaz, Universidad Autónoma de Madrid, Reyes Católicos 2, Madrid 28040 Spain |
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Abstract: | Incubation of rat splenic microsomes with the catalytic subunit of cyclic AMP-dependent protein kinase in the presence of Mg-ATP stimulated 2-3-fold lyso-platelet-activating factor:acetyltransferase activity. This activation was due to an increase in the of the acetylation reaction, whereas the for acetyl-CoA was not affected. The ATP derivative, AMPPNP, could not replace ATP and preincubation of the microsomes with the heat-stable inhibitor of protein kinase prevented the activation by Mg-ATP obtained in the presence of the protein kinase. Activation of the acetylation reaction by the protein kinase was reversible. Evidence is provided that the reversal of activation is due to dephosphorylation of the enzyme. These data provide evidence that in vitro lyso-platelet-activating factor:acetyltransferase from splenic microsomes is regulated by phosphorylation. |
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Keywords: | cyclic AMP Platelet-activating factor Protein kinase Acetyl-CoA transferase (Rat spleen microsome) PAF-acether platelet-activating factor |
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